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The 3rd generation lentiviral vector system consists of four oakleysunglasses 4920 3 quart pot plasmids4. The transfer vector offers the transgene or silencing cassette to get delivered within a lentiviral backbone containing each of the cis acting sequences meant for genomic RNA production and packaging. Lentiviral transfer vectors is often created to express (constitutively or conditionally) both transgenes and shRNAs in single units or multiple combinations.5, 6, 7, 8 Three additional plasmids (pMDL, pRev and pVSVG) give the trans acting factors required for packaging (for further details, see Fig.

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