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To assess the subcellular origin of natural A oligomers secreted by cells expressing human APP, we prepared microsomes from 7PA2 Chinese hamster ovary (CHO) cells, which stably express the V717F Alzheimer's mutation in APP751 (an APP:isoform 751 amino acids in total length)8, 10. A properly oakleysunglasses 004068 06 gti turbo sensitive immunoprecipitation/western blot method10 revealed both monomers and oligomers of the inside the isolated, washed microsomes, plus the A dimers within the microsomes co oakleysunglasses 004068 06 gti turbo migrated with those involved with the conditioned medium (CM) (Fig. 1a)..

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